EuroFlow PIDOT 2026
Resources
Detailed information
EuroFlow SOPs and relevant documents
EuroFlow SOP for sample preparation. Version 1.7 - November 2023
EuroFlow SOP for bulk lysis in MRD panels. Version 2.0 – June 2025
Composition and technical info of EuroFlow panels for PID. Version 1.2 - September 2022
EuroFlow SOP for instrument setup and compensation for DxFlex. Version 2.0 – February 2025
EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto II. Version 2.0 – February 2025
EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 2.0 – June 2025
EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.3 – February 2025
EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 – October 2019
Reference values for PB leukocyte populations in percentages (EuroFlow PIDOT). Version 1.2 - December 2024
Reference values for PB leukocyte populations in absolute numbers of cells (EuroFlow PIDOT). Version 1.1 - July 2024
The above documents can be accessed after creating a EuroFlow account and upon login at app.euroflow.org/login
Downloads for BD Lyric (if applicable):
Reagent files (zip) (download and extract) – June 2025
Assay files (zip) (download and extract) – June 2025
Label file (xml)
Videos
Educational video: EuroFlow SOP for instrument setup and compensation (for BD LSR II and BD FACS Canto)
euroflow.org
Publications
Neirinck et al., The EuroFlow PIDOT external quality assurance scheme: enhancing laboratory performance evaluation in immunophenotyping of rare lymphoid immunodeficiencies. Clin Chem Lab Med. 2024 Oct 21.
pubmed.ncbi.nlm.nih.gov
van der Burg et al., The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019 Mar 4;10:246.
pubmed.ncbi.nlm.nih.gov
van Dongen et al., EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019 Jun 13;10:1271.
pubmed.ncbi.nlm.nih.gov
Linskens et al., Improved Standardization of Flow Cytometry Diagnostic Screening of Primary Immunodeficiency by Software-Based Automated Gating. Front Immunol. 2020 Nov 2;11:584646.
pubmed.ncbi.nlm.nih.gov
Neirinck et al., The Euroflow PID Orientation Tube in the diagnostic workup of primary immunodeficiency: Daily practice performance in a tertiary university hospital. Front Immunol. 2022 Sep 13;13:937738.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A. 2015 Feb;87(2):145-56.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Frequent issues and lessons learned from EuroFlow QA. J Immunol Methods. 2019 Dec;475:112520.
pubmed.ncbi.nlm.nih.gov